Get easy, consistent transgenesis

At the heart of SBI’s PiggyBac Transposon System* is the Super PiggyBac Transposase, an engineered, hyperactive version of the enzyme that can efficiently integrate any-sized DNA insert into the genome.

Why use the PiggyBac Transposon System?

Easy, consistent transgenesis with no limits on cargo size—For transgenesis that’s easy, consistent, and not limited by cargo size, SBI’s PiggyBac Transposon System is an excellent choice. The system consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.

Footprint-free removal that leaves no PiggyBac sequences behind—In addition to ease-of-use, consistency, and the lack of limits on DNA insert size, what sets this system apart is the ability to reverse the integration reaction in a footprint-free way—with the Excision Only PiggyBac Transposase (Cat.# PB220PA-1), the ITRs and cargo that the Super PiggyBac Transposase integrates into the genome can be removed, leaving behind the original genomic sequence and nothing else.

• Make transgenic cell lines with a single transfection
• Integrate multiple PiggyBac Vectors in a single transfection
• Insert an expression cassette into human, mouse, and rat cells
• Deliver virtually any-sized DNA insert, from 10 – 100 kb
• Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
• Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (Cat.# PBC100A-1)

Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to evaluate the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.